Fig. 4From: Geranylgeranyl diphosphate synthase inhibitor and proteasome inhibitor combination therapy in multiple myelomaCombination treatment with RAM2061 and PI leads to induction of the ICD pathway markers. MM cells were incubated with RAM2061 (50 or 100 nM) for 48 h with or without sequential (added after 24 h, denoted by *) or concurrent PI treatment (Bor: 3 nM (MM.1S), 5 nM (JJN3 and RPMI-8226), 15 nM (ALMC-2), Carf: 20 nM). a Immunoblot analysis of secreted HMGB1 was performed using media collected from cells. b, c Cell surface levels of calreticulin were measured by flow cytometry. Data are expressed as the average percentage of calreticulin positive (n = 3, data are displayed as mean ± stdev, * denotes p < 0.05. ** denotes p < 0.01. *** denotes p < 0.001 per t-test). Blots are representative of three independent experimentsBack to article page