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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Geranylgeranyl diphosphate synthase inhibitor and proteasome inhibitor combination therapy in multiple myeloma

Fig. 3

Effects of sequential PI therapy in combination with RAM2061 on markers of the unfolded protein response. MM cells were incubated with RAM2061 (50 or 100 nM) for 48 h with or without sequential PI treatment. Bortezomib or carfilzomib was added either a 6 h (denoted by **) or b 24 h (denoted by *) prior to cell harvest (Bor: 3 nM (MM.1S), 5 nM (JJN3 and RPMI-8226), 15 nM (ALMC-2), Carf: 20 nM). Immunoblot analysis for ATF4, IRE1, p-eIF2α, and eIF2α was performed. β-tubulin is shown as a loading control. Blots are representative of three independent experiments

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