Fig. 3From: Geranylgeranyl diphosphate synthase inhibitor and proteasome inhibitor combination therapy in multiple myelomaEffects of sequential PI therapy in combination with RAM2061 on markers of the unfolded protein response. MM cells were incubated with RAM2061 (50 or 100 nM) for 48 h with or without sequential PI treatment. Bortezomib or carfilzomib was added either a 6 h (denoted by **) or b 24 h (denoted by *) prior to cell harvest (Bor: 3 nM (MM.1S), 5 nM (JJN3 and RPMI-8226), 15 nM (ALMC-2), Carf: 20 nM). Immunoblot analysis for ATF4, IRE1, p-eIF2α, and eIF2α was performed. β-tubulin is shown as a loading control. Blots are representative of three independent experimentsBack to article page