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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Geranylgeranyl diphosphate synthase inhibitor and proteasome inhibitor combination therapy in multiple myeloma

Fig. 1

Effects of sequential and concurrent bortezomib therapy in combination with RAM2061 on induction of apoptosis. MM cells were incubated with RAM2061 (50 or 100 nM) for 48 h. Bortezomib was added either concurrently or after 24 h (sequential; denoted by *) (Bor: 3 nM (MM.1S), 5 nM (JJN3 and RPMI-8226), 15 nM (ALMC-2)). Cells were stained with fluorescently conjugated Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Data are expressed as the average percentage of Annexin V+/PI− (early apoptotic) and Annexin V+/PI+ (late apoptotic) (n = 3, data are displayed as mean ± stdev, * denotes p < 0.05. ** denotes p < 0.01. *** denotes p < 0.001 per t-test)

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