Fig. 4

MiR-125b/STARD13 axis is a potential target of Tanshinone IIA. A MiR-125b level was measured in breast cancer cells treated with different concentration of Tanshinone IIA as indicated. B The correlation between the overall survival probability of breast cancer patients and miR-125b was examined through Kaplan – Meier (KM) Plotter analysis. C The correlation between the pathologic status of breast cancer patients and miR-125b level was examined through OncomiR analysis. D MiR-125b level was detected in breast cancer-derived stem cells and breast cancer cells via analyzing the GEO dataset (GSE97765). E MiR-125b level was detected in different types of breast cancer cells via analyzing the GEO dataset (GSE146477). F MiR-125b level was examined in MCF-7 spheroids and cells. G and H Expression of STARD13, a target of Tanshinone IIA was determined in cells depicted in A. I Luciferase activity of Luc-STARD13-3’UTR was examined in breast cancer cells with miR-125b overexpression plus Tanshinone IIA (10 μM). J MiR-125b level was evaluated in (I)-described cells. K, L STARD13 expression was examined in breast cancer cells with Tanshinone IIA (10 μM) as well as miR-125b overexpression or not. M MiR-125b level was detected in the cells depicted in F. n = 3, *p < 0.05, **p < 0. 01 vs. Control, ^##p < 0. 01 vs. Tanshinone IIA. For transfection experiments, control groups were transfected with miR-125b mimics NC