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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Tanshinone IIA attenuates the stemness of breast cancer cells via targeting the miR-125b/STARD13 axis

Fig. 1

Tanshinone IIA attenuates breast cancer stemness in vitro. A, B qRT-PCR was utilized to examine the mRNA levels of stemness markers (Oct4 and ALDH1A1) in Tanshinone IIA-treated breast cancer cells. C Tanshinone IIA-treated breast cancer cells were followed by examination of stemenss markers (Oct3/4 and ALDH1A1) protein levels through western blot. D, E Tanshinone IIA-mediated effects on spheroid formation were evaluated by measuring the spheroid size (D) and number (E). F, G Representative FACS profile of breast cancer cells were detected with CD44+ and CD24− markers and quantified. H ALDH activity was measured in Tanshinone IIA-treated breast cancer cells. I, J Cell viability was assessed in the cells described in A. **p < 0.01 vs. Control, n = 3

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Experimental Hematology & Oncology

ISSN: 2162-3619

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