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Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: Red blood cells differentiated in vitro using sequential liquid and semi-solid culture as a pre-clinical model

Fig. 2

In vitro differentiated RBCs grown in sequential culture show high CD235 positivity. a Timeline schematic of modified two-stage methodology. At day 8, cells are collected and plated in semi-solid medium containing EPO for an additional week. b Sample flow diagram for erythroid profiles after sequential culture systems. Cells are gated on live populations. RBC flow control is shown on right panel. c CD235 positivity in erythroid profiles after sequential culture system or only liquid culture system (p  = 0.0117)

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