Fig. 1

Sequential liquid culture and semi-solid media culture system produces robust erythroid response. a Timeline schematic of semi-solid medium plating schedule using the original two-stage methodology. Cell populations were plated in semi-solid medium containing EPO after each major end point: post-isolation, post-expansion, and post-differentiation. These are noted in red. b CD235/CD71 erythroid profiles of cells grown in semi-solid medium plated after major end points as indicated. c Wright-giemsa staining of enucleated erythroid cells grown in semi-solid medium (originally plated at post-expansion end point). d Quantification of CD235/CD71 erythroid profiles of semi-solid medium grown cells. e Quantification of live/dead staining of semi-solid medium grown cells