Fig. 2

C-NHEJ in V(D)J recombination process. A RAG proteins bind to the V(D)J sequence at DNA, leading to DNA cleavage, creating DSB. Afterward, Ku70/80 heterodimers locate and bind to the DSB end. B Ku70/80 directly recruits DNA-PKcs. Following DNA-PKcs autophosphorylation, it activates the main nuclease in c-NHEJ, Artemis. C After trimming of DNA ends by Artemis, DNA polymerases reconstruct the DNA. Consequently, the Ku-DNA complex anchors PAXX, XRCC4, XLF, and Lig IV to rejoin the DNA ends. D Fully functional recombined DNA is ready to be translated