Fig. 2

Immune regulation associated with wild-type and JAK2V617F mutant cell competition. A A murine model of wild-type and JAK2V617F mutant cell competition established by marrow transplantations. B Differentially enriched Gene Ontology (GO) terms in mutant Lin⁻cKit⁺ HSPCs transplanted together with wildtype cells (pooled sample from 3 mice) compared to mutant HSPCs transplanted alone (pooled sample from 2 mice). P values are plotted as the negative of their logarithm. C Spleen T cells (CD3⁺CD4⁺and CD3⁺CD8⁺) and B cells (CD3⁻B220⁺), and marrow MDSCs (both CD11b⁺Ly6ChighLy6G⁻ M-MDSCs and CD11b⁺Ly6ClowLy6G⁺PMN-MDSCs) in wild-type recipient of wild-type donor (“wild-type”), JAK2V617F mutant donor (“mutant”), or both wild-type and mutant donors (“wild-type + mutant”) (spleen T cells: n  =  3 mice in each group; spleen B cells: n  =  3–6 mice in each group; marrow MDSCs: n  =  3–6 mice in each group). D Quantitative measurement of mean fluorescence intensity for PD-L1 staining on wild-type LSK cells (n  =  7 mice), JAK2V617F mutant LSK cells (n  =  4 mice), and JAK2V617F mutant LSK cells with co-existing wild-type cell competition (n  =  9 mice). *P  <  0.05