Fig. 2
Siglec-10hi cells are enriched in HCC and prominently distributed on CD68+ TAMs. a Representative images showed Siglec-10hi cells in tumor and peritumor tissues. b HCC tissues were co-labeled with Siglec-10 (red) and macrophage marker CD68 (green). Nuclei were counterstained blue with DAPI. The lower right panel was merged by the aforementioned three images. c, d Representative flow cytometry figures (c) and cumulative results (d) showing subpopulations gated on Siglec-10hi CD45+ leucocytes from fresh human HCC samples (n = 20). Bars represent the mean ± standard deviation. Data were analyzed using the Student’s t-test. e, f Representative images (e) and cumulative results (f) of flow cytometry showed infiltration of Siglec-10hi CD68+ cells in total CD45+ CD68+ cells in tumor and peritumor tissues (n = 14). Data were analyzed using the Student’s t-test