Fig. 8

Immunohistochemical staining for CD80, FLI1, and IL21R between melanoma and normal skin tissues. a FLI1 binding sites and downstream targets, including CCL3, CD28, HLA-G, and PTPRC were identified. Docking quality of the model was confirmed by HDock. b FLI1 expression and its targets after FLI1 knockdown, under co-culture conditions. c The protein levels of secreted CCL3 following FLI1 knockdown. d Melanoma cell viability when co-cultured with TAMs; detected by a CCK-8 assay. e The migration of melanoma cells after co-culturing with TAMs; detected by a wound healing assay. f The invasion of melanoma cells after co-culturing with TAMs, detected by a transwell invasion assay. g Schematic diagram of TAMs’ role in the TME. h Top: FLI1 expression levels in melanoma and normal tissue; bottom: FLI1 expression levels in higher and lower immunity groups. “High” stands for the higher immunity group and “low” stands for lower immunity group