Fig. 6

Mcl-1 overexpression alleviates apoptosis induced by the combination of venetoclax and ATO in KG1a cells. a, Western blot analysis of indicated proteins in KG1a cells treated with venetoclax (200 nM), ATO (3 µM), or both in combination for 48 h after transfection with pcDNA3.1 control or pcDNA3.1-Mcl-1 vector. b, c, Representative flow cytometric analysis (b) and summary data (c) of the percentage of Annexin V⁺PI⁺ apoptotic cells in KG1a cells transfected with pcDNA3.1 control or pcDNA3.1-Mcl-1 vector after treatment with venetoclax (100 nM), ATO (3 µM), or both in combination for 48 h. Values were obtained from two independent experiments, and horizontal bars indicate mean ± s.d. *P values versus control treatment by two-tailed Mann–Whitney U test. n.s., not significant