Table 1 Effect of platelet desialylation in thrombocytopenia
From: Platelet desialylation and TFH cells–the novel pathway of immune thrombocytopenia
Reference | Finding | Methodology |
|---|---|---|
Grodzielski [46] | Desialylation↑in the presence of GPIb-positive ITP | CD34 + cells were incubated with ITP or control plasma |
Dupont [63] | Type 2B VWD and mice carrying the p.V1316M mutation elevated desialylation | Samples from patients and mice with type 2B VWD were collected |
Jansen [70] | Refrigeration↑→desialylation↑, DANA inhibited desialylation, Desialylation↑→GPIb, GPV shedding↑ | Platelets stored in DANA or PBS, fresh or refrigerated at 4 °C, were injected into mice |
Kullaya [74] | Desialylation↑→ADP signaling ↑→NanA exposure↑→platelet hyperactivity | Whole blood or PRP was incubated with 200 mU purified neuraminidase and supernatants with and without NanA |
Qiu [37] | CD8 + T cells ↑→phagocytosis↑ DANA inhibits phagocytosis | CD8 + T cells and platelets were cocultured, and DANA was added |
Grozovsky [73] | Platelets desialylation↑→AMR→JAK2/STAT3↑→TPO mRNA↑ | WT or Asgrs/st3gal4- mice were treated with sialydase and RAMPs |
Hinek [83] | NEU1→PDGF/IGF receptors→PDGF and IGF↓→arterial SMCs↓ | AoSMCs were treated with ddNANA, anti-neuraminidase and preimmune IgG |
Ma [79] | SLC35A1 mutations→sialylation↓→megakaryocyte↓, megakaryocyte maturation↓, platelets clearance in liver↑ | Mouse line with the Slc35a1 gene was established |