Fig. 3

let-7b targets and down regulates an AML1-ETO 3’UTR reporter in HEK293T cells. a Schematic representation of miRNAs with predicted target sites within the 2800–3400 bp AML1-ETO 3′UTR fragment. Predictions were made using TargetScan 7.2. b miRNA mimics or non-targeting control mimic were co-transfected into HEK293T cells with the AML1-ETO 3.7 kb 3′UTR luciferase reporter and luciferase assays were performed 24 h post-transfection. Data is shown as relative luciferase activity normalized to non-targeting control mimic. Significance was determined using one-way ANOVA with a post-hoc Tukey test