Fig. 2

AML1-ETO 3′UTR cis-elements affect expression and are targeted by miRNAs. a Schematic of the dual luciferase reporters containing 600 bp fragments of the AML1-ETO 3′UTR. b Individual AML1-ETO 3′UTR dual luciferase reporters were transfected into Kasumi-1 and SKNO-1 cells and luciferase activity was measured. Assay results were normalized to an empty vector control. Values and error bars represent the average and SD of relative luciferase from three independent experiments. Significance is shown compared to empty vector control using one-way ANOVA with a post-hoc Tukey test. c Representative western blot and quantification of AML1-ETO protein in SKNO-1 DICER knockdown or control shRNA cell lines. Values and error bars represent the mean and SD of three independent experiments. d SKNO-1 cells expressing shRNAs targeting DICER or control shRNA were transfected with dual luciferase reporters expressing the indicated AML1-ETO 3′UTR fragments. Values and error bars represent the mean and SD of relative luciferase activity of shDICER compared to shCTRL from indicated number of independent experiments. Significance is shown compared to shCTRL control using unpaired t-tests corrected for multiple comparisons using the Holm-Sidak method