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Table 1 The growth and experiment media for HMCLs

From: Targeting phosphoglycerate dehydrogenase in multiple myeloma

HMCLs

Growth medium

Experiment medium

IH1 and OH2

10% HS in RPMI + IL-6 (sup.)

2% HS in RPMI + IL-6 (1 ng/ml)

INA6 and ANBL6

10% FCS in RPMI + IL-6 (1 ng/mL)

2% HS in RPMI + IL-6 (1 ng/ml)

KJON1

5% HS in RPMI + IL-6 (sup.)

2% HS in RPMI + IL-6 (1 ng/ml)

JJN3 and AMO1

10% FCS in RPMI

2% HS in RPMI

RPMI8226

20% FCS in RPMI

2% HS in RPMI

U266

15% FCS in RPMI

2% HS in RPMI

  1. IL-6 (Gibco® by life technologies™)
  2. IL-6 sup: Cytokine mixture that includes IL-6, produced by monocytes upon activation with lipopolysaccharide
  3. HS heat-inactivated human serum (Blood Bank, St. Olav's Hospital), FCS Fetal calf serum (Gibco® by life technologies™), RPMI RPMI 1640 supplemented with 0.68 mM of L-glutamine (both from Sigma-Aldrich, St. Louis, MO, USA)