Skip to main content

Table 1 The growth and experiment media for HMCLs

From: Targeting phosphoglycerate dehydrogenase in multiple myeloma

HMCLs Growth medium Experiment medium
IH1 and OH2 10% HS in RPMI + IL-6 (sup.) 2% HS in RPMI + IL-6 (1 ng/ml)
INA6 and ANBL6 10% FCS in RPMI + IL-6 (1 ng/mL) 2% HS in RPMI + IL-6 (1 ng/ml)
KJON1 5% HS in RPMI + IL-6 (sup.) 2% HS in RPMI + IL-6 (1 ng/ml)
JJN3 and AMO1 10% FCS in RPMI 2% HS in RPMI
RPMI8226 20% FCS in RPMI 2% HS in RPMI
U266 15% FCS in RPMI 2% HS in RPMI
  1. IL-6 (Gibco® by life technologies™)
  2. IL-6 sup: Cytokine mixture that includes IL-6, produced by monocytes upon activation with lipopolysaccharide
  3. HS heat-inactivated human serum (Blood Bank, St. Olav's Hospital), FCS Fetal calf serum (Gibco® by life technologies™), RPMI RPMI 1640 supplemented with 0.68 mM of L-glutamine (both from Sigma-Aldrich, St. Louis, MO, USA)