Fig. 1

HMCLs express PHGDH and are sensitive to its inhibition. a The expression of PHGDH, PSAT1, and PSPH was assessed by WB in eight HMCLs. b The chemical structure of both PHGDH inhibitors, CBR5884 and NCT-503. c, d CellTiter-Glo was used to assess HMCLs’ sensitivity to both PHGDH inhibitors, CBR5884 and NCT-503, respectively. The cells were treated with indicated doses overnight. e Proliferation of HMCLs after an overnight incubation were incubated overnight with or without serine. f Proliferation of INA6 and U266 cells were treated overnight with different doses of NCT-503 in the presence or absence of serine. g INA6 cells were treated overnight with their corresponding IC50 × 3 doses of CBR5884 or NCT-503, and then apoptosis was assessed by flowcytometry using Annexin-V/PI assay. h INA6, RPMI8226 and U266 cells were treated for 4 h with double their corresponding IC50 of NCT-503, and then apoptotic proteins were investigated by WB. All the presented graphs and calculated IC50s represent three independent experiments with minimum two replicates. Error bars are ± SEM and *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001