Fig. 4
IL-6 knockdown in ssCART-19 cells preserves antitumor efficacy in vivo. a Schematic of the Raji-luc xenograft model. NOD/SCID/γc-/- (NSG) mice were injected via the tail vein with 1 × 106 Raji-luc cells on day 0. Bioluminescence imaging (BLI) was performed on day 1 to quantify engraftment. The mice were then randomized and treated with freezing medium (n = 3), untransduced T cells (n = 3), 6 × 106 regular CART-19 cells (n = 3), or 6 × 106 ssCART-19 cells (n = 3). b Images showing BLI of tumor growth in mice. Mice treated with both ssCART-19 cells and regular CART-19 cells showed obviously tumor inhibition, however, rapidly tumor progression were observed in the mice treated untransduced T cells, or freezing medium. c Survival of mice treated with regular CART-19 cells, ssCART-19 cells, untransduced T cells, or freezing medium