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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Interleukin-6-knockdown of chimeric antigen receptor-modified T cells significantly reduces IL-6 release from monocytes

Fig. 3

IL-6 knockdown in ssCART-19 T cells reduces IL-6 release from monocytes. a IL-6 mRNA levels and b IL-6 protein levels in ssCART-19 cells and regular CART-19 cells after stimulation with Raji. Differences were assessed for significance using one-way ANOVA. Data are expressed as the mean + SD. NS, no significant difference, ***p < 0.001. c IL-6 levels in the medium of primary monocyte cultures incubated with supernatants from regular CART-19 or ssCART-19 cells cocultured with Raji cells. Differences were assessed using one-way ANOVA. NS, no significant difference. **p ≤ 0.01; ***p ≤ 0.001, ****p ≤ 0.0001. d IL-6, e IFN-γ and f IL-2 protein levels in the supernatants of ssCART-19 or regular CART-19 cells cocultured with Raji cells. The cells were re-stimulated every 36 h as indicated by the black arrows. Differences were assessed for significance using a paired T test. ***p < 0.001

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