Fig. 6

Rescue RelB in G9a depleted MM cells eliminates inhibition of proliferation. a Immunoblots show expression levels of G9a and RelB in KMS12BM transfected with lentiviruses expressing shNT or shG9a and RelB recovered G9a depleted KMS12BM cells after long term culture. b Proliferation assay on RelB recovered G9a-depleted JJN3 cells with control JJN3 cells infected by lentiviruses expressing shNT. Luminescent cell viability assay was conducted with CellTiter-Glo^® at indicated time points. The viability at day0 was measured as 1 and following cell viability was normalized accordingly. Two-tailed Student’s t test, *p < 0.05, for comparisons of shNT, \(RelB^{ + }\) shG9a#1 and \(RelB^{ + }\) shG9a#2 versus shG9a. c Tumor volumes over time of KMS12BM xenografts generated with shNT transfected or shG9a transfected/RelB-recovered KMS12BM cells. (n = 3, mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 for comparisons of shNT, \(RelB^{ + }\) shG9a#1 and \(RelB^{ + }\) shG9a#2 versus shG9a, Bonferroni corrected one-way ANOVA). d Final individual and mean tumor volumes of KMS12BM xenografts generated with shNT transfected or shG9a transfected/RelB-recovered KMS12BM cells at day 31. (Bonferroni corrected one-way ANOVA comparing all groups, **p < 0.01, ****p < 0.0001)