Fig. 3
From: Frequent upregulation of G9a promotes RelB-dependent proliferation and survival in multiple myeloma
G9a depletion inhibit proliferation of MM cells in vitro. Proliferation assay on G9a-depleted MM cells with control cell lines infected by lentiviruses expressing shNT. KMS12BM (a) and JJN3 (b). Luminescent cell viability assay was conducted with CellTiter-Glo® at indicated time points. The viability at day0 was measured as 1 and following cell viability was normalized accordingly. Two-tailed Student’s t test, *P < 0.05, **P < 0.01 and ***p < 0.001. c KMS12BM and JJN3 cells were infected with lentiviruses expressing shNT or shG9a and 24 h later equal number of cells were seeded into semisolid methyl-cellulose cultures with G9a/GLP inhibitor UNC0642 treated cells as positive control. Colony formation was followed for on day 14 and the images and numbers of colonies were presented as indicated. d Quantification of the colony formation assay. On day 14, colonies with size bigger than 100 cells were counted. N = 2, data shown are mean ± S.D. *P < 0.05, **P < 0.01 and ***p < 0.001