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Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: Frequent upregulation of G9a promotes RelB-dependent proliferation and survival in multiple myeloma

Fig. 2

G9a depletion and G9a/GLP inhibitor UNC0642 reduce histone methylation at H3K9me2 and H3K9me1. a Immunoblots show G9a and β-actin (loading controls) in indicated MM cell lines infected with lentiviruses expressing Non-targeting (NT) ShRNA (shNT) or sh-RNAs targeting EHMT2 (shG9a). b mRNA expression of EHMT2 in indicated MM cell lines infected with lentiviruses expressing shNT or shG9a (n = 3; mean ± SD; Bonferroni corrected one-way ANOVA compared with Non-targeting control; **P < 0.01 and ***p < 0.001). c Immunoblots show HEK9me2, H3k9me1 and H3 (loading controls) in (i) KMS12BM and (ii) JJN3 cells infected with lentiviruses expressing shNT or shG9a. d (i) Immunoblot analyses shows H3K9me2, H3K9me1 and H3 (loading control) in DMSO (control) or UNC0642 treated KMS12BM cells; 72-h treatment of UNC0642 at \(IC_{50}\). (ii) Dose–response curves and \(IC_{50}\) of UNC0642 on KMS12BM cells infected with lentiviruses expressing shNT or shG9a. Data are mean ± SD of three independent biological replicates (n = 3; mean ± SD; ***p < 0.001)

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