Fig. 1
From: The SMAC mimetic LCL-161 selectively targets JAK2V617F mutant cells
JAK2V617F mutant cells lines are more sensitive to killing by LCL-161 under certain circumstances. a–c. L929 cells expressing JAK2WT, JAK2V617F or empty vector were incubated with increasing concentrations of a LCL-161 alone, b LCL-161 with the addition of 0.25 ng/ml mTNFα, c LCL-161 with the addition of 400 ng/ml mTNFa neutralizing antibody for 48 h and then analyzed with a resazurin based viability assay. ****P < 0.0001, 2way ANOVA. d Western blot of L929 cell lines harvested 24 h after exposure to combinations of LCL-161 and mTNFα. e–g HEL and K562 cells were incubated with e LCL-161 alone, f LCL-161 + 1 ng/ml hTNFα, or g LCL-161 + 10 ng/ml hTNFα for 48 h. Apoptosis was measured with Annexin V and PI staining. **P < 0.01, ****P < 0.0001 unpaired t test