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Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: A high-throughput screening identifies histone deacetylase inhibitors as therapeutic agents against medulloblastoma

Fig. 2

Dacinostat and quisinostat increase apoptosis of Daoy and D283 cells. a, b Daoy and D283 cells were treated with dacinostat (0.1 µM for Daoy cells, 0.01 µM for D283 cells) or quisinostat (0.4 µM for Daoy cells, 0.04 µM for D283 cells) for 24 h and 48 h, respectively. Apoptosis was analyzed with flow cytometry. Experiments were performed three times with one representative scatterplot shown. c, d Percentage of apoptotic cells. An unpaired t test was performed to compare each treatment group with the vehicle control (PBS). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. e, f Dacinostat and quisinostat induce the cleavage of caspase-3 and PARP. Daoy and D283 cells were treated with dacinostat and quisinostat at indicated concentrations for 24 h and 48 h, respectively. The target protein expression was analyzed by western blot

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