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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Novel GTF2IPDGFRB and IKZF1TYW1 fusions in pediatric leukemia with normal karyotype

Fig. 3

Molecular genetic and FISH analyses for identification of the IKZF1TYW1 fusion gene. a The sequence which was obtained using the FusionCatcher software with the fastq files of the RNA sequencing data. b Partial sequence chromatogram of the PCR amplified cDNA fragment showing the fusion (arrow) of IKZF1 and TYW1. c Ideogram of chromosome 7 showing the mapping position of IKZF1 (vertical red line) and TYW1 (vertical green line). d Diagram showing the FISH probe (RP11-813K3 and RP11-95E2) for IKZF1. Additional genes in this region are also shown. e Diagram showing the FISH probe (RP11-458F8 and RP11-166O4) for TYW1. Additional genes in this region are also shown. f FISH on interphase nuclei with the IKZF1 (red signal) and TYW1 (green signal) probes showing a red signal, a green signal, and two yellow-fusion signals (arrow)

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