Fig. 3

Pattern of DNA methylation of the miR-130b/miR-301b locus in prostate tissue of TCGA-PRAD. a Methylation levels (beta-value) of the 12 CpG dinucleotide probes located along the gene obtained using the Infinium HumanMethylation450 BeadCHiP array. The beta-value of methylation of each site from 50 normal and 50 matched tumor samples is plotted as grey (normal) and black (tumor) circles. Mean and standard deviation of the measurements are presented. The ratio of fluorescence intensity between the unmethylated and methylated sites ranges between 0 and 1 respectively. Horizontal boxes indicate the position of the CpG island, S-shore, precursor miRNAs and POLR2A (RNA Polymerase II). Data for cg16244770 and cg03636163 is not available at TCGA-PRAD. b Relationships between fold change (FC) in DNA methylation and miR-130b expression in matched normal vs tumor tissue (n = 35), r 0.4736, p 0.0041 c Relationships between DNA methylation and miR-130b expression in all available samples (n = 241), r 0.5242 and p < 0.0001. The non-parametric Spearman correlation coefficient (r) is indicated. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns non-significant