Fig. 4
From: Microfluidic device for primary tumor spheroid isolation
Mouse macrophage-specific marker Emr1 is highly expressed in the captured spheroids (a), and fibroblast-specific marker α-SMA is also moderately expressed in the captured spheroids (b). For Emr1 expression, KPC pancreatic tumor cells were used as a negative control, and murine bone marrow macrophages were used as a positive control. For α-SMA expression, KPC pancreatic tumor cells were used as a negative control, and murine NIH-3T3 fibroblasts were used as a positive control. The relative mRNA expression of Emr1 and α-SMA were measured by RT-qPCR