Fig. 3
From: Microfluidic device for primary tumor spheroid isolation
The trapped spheroids carried the Kras G12D point mutation. According to the sequencing map of the amplified Kras codon 12 (exon 1), both the A T C and A C C sequences are present at the site of mutation (the T-peak is equal to the C-peak). Therefore, the complementary sequence corresponds to G A T and G G T, which represents the mutated Kras G12D and the wild-type Kras sequences, respectively