Fig. 4
From: PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle
PTEN silencing induces chromosomal instability. a HeLa cells seeded in chamber slides were transfected with siRNAs to PTEN or luciferase for 48Â h, after which cells were fixed and stained with antibodies to tubulin and CREST. DAPI was also used to label DNA. Stained cells were examined under a fluorescent microscope and representative images are shown. b, c Percentages of normal or abnormal mitotic cells as shown in a were summarized and presented