Fig. 5

REH-CM increases SA-βGAL activity in the BM-MSC. a Representative SA-β-gal staining of BM MSC isolated from the NN10, NN1 and LN. b The chemotactic effect of SDF-1 (100 ng/ml) on CD34+ HSC migration in the different niches was studied using a modified Boyden chamber assay. Results are expressed as the fold increase migration from two independent experiments done in duplicates. (ns: non-significant, *p < 0.05, **p < 0.01). c Analysis of a multiplexed cytometric bead assay (CBA) of the LN. Cytokines (IL-8, IL-1B, IL-6, IL-10, TNFα and IL-12p70) concentration in the samples was calculated from the data obtained by the FACS array