Fig. 4

HSC from the LN have abnormal gene expression of molecules involved in self-renewal. RT-qPCR analysis of a Ezh2, b c-Kit, c Klf4 gene expression in HSC co-cultured with MSC and REH-CM. Results are normalized to the housekeeping gene RPS18. Graphs show SEM; n = 3, ns: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001 were calculated by using Student’s t test in GraphPad Prism5