Fig. 2

Lentiviral transduction of c-Kit restores the defects in colony forming units in Pim1^−/− and Pim TKO BM cells. BM cells isolated from WT, Pim1^−/−, Pim2^−/−, or Pim TKO mice were transduced with control lentiviruses (Fu-CRW-Ctl) or c-Kit-expressing lentiviruses (Fu-CRW-c-Kit) as described in “Methods and materials” section. The transduced cells were plated in Methocult M3434 for colony forming assay (30,000 cells/well) on 6-well plates. CFUs (CFU-GM, BFU-E and CFU-GEMM) were quantified and shown. (*p<0.05, ** and ^##p<0.01). Data represent 3 independent experiments