Fig. 4
From: Activation of NQO1 in NQO1*2 polymorphic human leukemic HL-60 cells by diet-derived sulforaphane
Effect of SFN on the changes of NFκB p65, p50, IκB, bcl-2 and bax expression in HL-60 cells. a Western blot analysis revealed the alteration on NFκB subunits p65/p50 and IκB expression in 48 h SFN treated cells. b The total protein expression level of bax and bcl-2 was determined by Western blot analysis and the normalized level of cellular bax and bcl-2 and alteration in the ratio of bax to bcl-2 in HL-60 cells treated for 48 h with increasing doses of SFN were calculated. In a and b, the intensity of the specific immunoreactive bands were quantified by densitometry and expressed as a fold difference against actin (loading control)