Fig. 1

Effect of SFN on NQO1 protein expression and activity. a Western blot analysis of NQO1 protein expression in cell lysates treated with 0, 1, 2.5, 5, 10 and 25 μM SFN for 24 and 48 h. b NQO1 enzyme activity assay in cell lysates treated with 0, 1, 2.5, 5, 10 and 25 μM SFN for 24 and 48 h. c Subcellular distribution of Keap 1 and Nrf2 in the cytosol, mitochondria and nucleus in control and 48 h, SFN-treated HL-60 cells. Actin and histone H1 were used as loading controls for cytosolic and nuclear fractions, respectively. The intensity of specific immunoreactive bands was quantified by densitometry and expressed as fold differences against actin. Values of NQO1 enzyme activities are mean ± SD for three experiments and activities of NQO1 are expressed as µmol of NADPH oxidized·min⁻¹