Fig. 2

No synergy in transforming capacity of NPMc⁺ and Meis1 or Hoxa9. a Percentage of single and double transduced cells forming colonies on methocult plates after serial replatings: Control (empty GFP+/YFP+ vector) (n = 4 at replate 1 and n = 2 at replate 2), NPMc+ (n = 7 at replate 1 and 2 and n = 5 at replate 3), Meis1 (n = 3), Hoxa9 (n = 10 at replate 1 and 2, n = 7 at replate 3), Meis1-NPMc⁺ (n = 3), and Hoxa9-NPMc⁺ (n = 4 at replate 1 and 2 and n = 2 at replate 3). b–f Representative images from May-Grünewald-Giemsa staining showing NPMc⁺ cells (b), Meis1 cells (c), Hoxa9 cells (d), Meis1-NPMc⁺ cells (e) and Hoxa9-NPMc⁺ cells (f). g The percentage of NPMc⁺ , Meis1, Hoxa9, Meis1-NPMc⁺ and Hoxa9-NPMc⁺ cells (reflected by GFP+/YFP+ expression) in peripheral blood was monitored by flow cytometry during 21 weeks. NPMc⁺ (n = 8), Meis1 (n = 5), Hoxa9 cells (n = 5), Meis1-NPMc⁺ cells (n = 5), and Hoxa9-NPMc⁺ (n = 10). Error bars show ± standard error of the mean (SEM)