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Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: Mutated NPM1 in combination with overexpression of Meis1 or Hoxa9 is not sufficient to induce acute myeloid leukemia

Fig. 2

No synergy in transforming capacity of NPMc+ and Meis1 or Hoxa9. a Percentage of single and double transduced cells forming colonies on methocult plates after serial replatings: Control (empty GFP+/YFP+ vector) (n = 4 at replate 1 and n = 2 at replate 2), NPMc+ (n = 7 at replate 1 and 2 and n = 5 at replate 3), Meis1 (n = 3), Hoxa9 (n = 10 at replate 1 and 2, n = 7 at replate 3), Meis1-NPMc+ (n = 3), and Hoxa9-NPMc+ (n = 4 at replate 1 and 2 and n = 2 at replate 3). bf Representative images from May-Grünewald-Giemsa staining showing NPMc+ cells (b), Meis1 cells (c), Hoxa9 cells (d), Meis1-NPMc+ cells (e) and Hoxa9-NPMc+ cells (f). g The percentage of NPMc+ , Meis1, Hoxa9, Meis1-NPMc+ and Hoxa9-NPMc+ cells (reflected by GFP+/YFP+ expression) in peripheral blood was monitored by flow cytometry during 21 weeks. NPMc+ (n = 8), Meis1 (n = 5), Hoxa9 cells (n = 5), Meis1-NPMc+ cells (n = 5), and Hoxa9-NPMc+ (n = 10). Error bars show ± standard error of the mean (SEM)

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