Fig. 2

PCM mice exhibit massive hepatosplenomegaly, extramedullary hematopoiesis, and increased colony growth of splenocytes. a Spleen and b liver weight [relative to total body weight (bwt)] in PCM (n = 5), PM (n = 5), CM (n = 5), and Ctrl (n = 5) mice 3 weeks after pI–pC injections. c Colony-forming ability of splenocytes isolated from PCM (n = 3), PM (n = 3), CM (n = 3) and Ctrl (n = 3) mice around 3 weeks after pI–pC injections. GEMM colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte, GM colony-forming unit-granulocyte, macrophage, E burst-forming unit-erythroid. d Representative flow cytometry plots of splenocytes with antibodies recognizing CD11b, Gr-1, c-Kit, Sca1 and Lineage-negative. The mean percentage of double-positive splenocytes from PCM (n = 3), PM (n = 3), CM (n = 3), and Ctrl (n = 3) mice is indicated. e Percentage of LSK cells in bone marrow from PCM (n = 3), PM (n = 3), CM (n = 3), and Ctrl (n = 3) mice, as determined with flow cytometry. f Colony-forming ability of bone marrow from PCM (n = 3), PM (n = 3), CM (n = 3), and Ctrl (n = 3) mice. g Western blots of protein extracts from splenocytes of PCM (n = 2), PM (n = 2), CM (n = 2), and Ctrl (n = 2) mice at the 3rd week after pI–pC injection. Actin was used as the loading control