| |
DMSO
|
Sirolimus
|
P
|
|---|
|
Mouse weight on Day 0 (g)
|
19.8 ± 4.3 (13)
|
19.0 ± 4.8 (13)
|
0.315
|
|
Thymus weight (mg)
|
39.2 ± 10.2 (13)
|
20.7 ± 6.9 (13)
|
<0.001
|
|
Thymocyte respiration
|
0.37 ± 1.0 (6)
|
0.36 ± 1.0 (6)
|
0.937
|
|
Thymocyte GSH (nmol mg−1)
|
44 ± 14 (9)
|
82 ± 45 (9)
|
0.019
|
|
Thymocyte caspase activity*
|
28 ± 15 (9)
|
12 ± 9 (9)
|
0.019
|
- The thymus was excised from C57BL/6 mice after intraperitoneal treatments with DMSO (0.5 µL/g on Days 0–3) or sirolimus (2.5 µg/g on Days 0–3). On Day 4, the weight of the entire gland was measured and fragments of the gland were processed for measuring cellular respiration, caspase activity and GSH level as described in “Methods” section
- Expressed as µM O2 min−1 mg−1
- * Expressed as AMC peak area (in arbitrary unit ÷103) mg−1
