Fig. 1

In vitro effects of sirolimus and everolimus on thymocyte respiration. a and c: One representative from 10 to 20 separate experiments. Each run represented a thymus fragment that was collected from a C57BL/6 mouse and processed immediately for measuring cellular respiration in the presence of 10 μM sirolimus (or everolimus) or 1.6 µL DMSO. The rate of respiration (k, μM O₂ min⁻¹) was the negative of the slope of [O₂] vs. t. The values of k _c (μM O₂ min⁻¹ mg⁻¹) are shown at the bottom of each run. The lines are linear fit. b and d: Summary of all results; the horizontal lines are mean