Fig. 5

Intron reintroduction within the wtCD20 coding sequence. a Schematic representation of position of alternative splice sites (ASS) in the wtCD20 coding sequence and within the constructs after reintroduction of introns 5 (CD20-int5), 3 and 6 (CD20-int3–int6), and 5 and 6 (CD20-int5–int6). ASS positions for D657 (closed circle), D618 (closed star), D480 (closed square), D393 (*), and D177 (closed triangle) are indicated. Gray and black symbols represent canonic and cryptic splice sites, respectively. b Specific RT-PCR detection of different CD20 variants in transfected HT1080 cell lines with different constructs. Plasmid was used as positive control and untransfected HT1080 cells as negative (ϕ). Raf amplification PCR was used as control for the cDNA synthesis