Fig. 4

RT-PCR and RT-qPCR assays of different CD20 transcript variants. a Full-length PCR (fl-CD20) allowed amplification of all CD20 alternative transcripts. Genomic DNA (gDNA) from wild-type PG13 (ø) and PG13 transfected by wtCD20, D657-, D618-, D480-, D393-, or D177-CD20 were amplified using primers specific for the 5′ (start codon) and 3′ (stop) CD20 gene regions, common to the six transcripts. H₂O was used as negative control (−), and the plasmid carrying the specific CD20 variant was added to the positive control (+). b CD20 variant-specific PCR was designed to amplify each alternative transcript specifically. c Proportion (in %) of each CD20 variant transcript in four different B cell lines. Means and SD calculated from seven independent experiments of RT-qPCR quantification