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Fig. 5 | Experimental Hematology & Oncology

Fig. 5

From: miR-199b, a novel tumor suppressor miRNA in acute myeloid leukemia with prognostic implications

Fig. 5

miR-199b silencing in vivo via a transduce and transplant approach. a Bone marrow transplant (BMT) approach schematic to delete miR-199b in hematopoietic cells. Mice were treated with 5-flourouracil, LSK (LinSca+Kit+) cells were obtained and silenced with an anti-miR-199b lentivirus. These cells were then transplanted in irradiated Ly5.1 mice. b Left panel: Lentiviral particles for anti-miR-199b-5p and control vector were used to transduce mouse HSCs. Silencing of miR-199b levels were confirmed in HSCs post transduction via RTqPCR. Right panel: Engraftment efficiencies post BMT were measured using staining for Ly5.2 versus Ly5.1 via flow cytometry. A representative flow cytometry data is shown from the analysis. cg Peripheral blood analysis for control and low-miR-199b transplant mice (n = 5). h Flow cytometry of peripheral blood B (CD19 staining) and T (CD3 staining) cells in control and low-miR-199b transplant mice at 27 weeks

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