Fig. 1

Intronic miR-199b expression in steady state hematopoiesis and its myelopoietic and hematopoietic potential. a miR-199b’s location within the genome; intronic of the DNM1 gene. b Human primary BM CD34⁺, BM CD33⁺, PB monocytes, PB eosinophils, and PB basophils expression of miR-199b in steady state hematopoiesis. c Lentiviral particles for anti-Has-miR-199b-5p and control vector were used to transduce human BM derived CD34 + cells. Silencing of miR-199b levels were confirmed in HSCs post transduction via RT-qPCR. d Lentiviral particles for anti-hsa-miR-199b-5p and scrambled/control vector were used to transduce human BM derived CD34 + cells. CFU-GM assay was performed and representative photomicrographs of the colonies are shown