Fig. 2

Detection of CIKs, CTLs and intracellular IFN-γ in T cells by flow cytometry. a On Day 10, the proportions of CD3+ CD56+ T cells were used to detect cytokine-induced killer cells (CIKs). And on Day 12, the proportion of CD3+ CD8+ T cells was used to identify cytotoxic T-lymphocyte cells (CTLs). The position of the gate was set by Isotype control. b The dot plots showed the proportion of T cells expressing IFN-γ. The left plot represented isotype control group, the middle plot represented Ad-Blank group, and the right plot represented the production of cytokines by DC induced antigen-specific CTL cells, and DC cells were infected by the recombinant non-proliferative adenovirus carrying the expression cassettes of TAAs