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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Therapeutic implications of intratumor heterogeneity for TP53 mutational status in Burkitt lymphoma

Fig. 1

Clinical history, therapeutic interventions and TP53 sequencing results. ad Immunohistochemistry slides showing Burkitt lymphoma medium-sized cells (Giemsa stain) expressing CD20 (b) and CD10 (c) (×400); c-MYC positivity in the inset (c). Peritoneal fluid collected at the moment of initial diagnosis (d), showing monomorphic BL cells with frequent mitotic figures. e FISH analysis of cells from peritoneal fluid using the Whole Chromosome Painting (WCP) probes of chromosomes 8 and 22, respectively pseudo-colored in red and green. The results showed the occurrence of the recurrent t(8;22)(q24;q11) translocation. fi Clinical course of the patient depicted over a 4 months period with time points of CT scans performed at initial diagnosis (f), after a first chemotherapy cycle (g), and at disease progression (h), and different therapeutic interventions (i). j, k TP53 deep targeted sequencing study of cells from the bulky mass and peritoneal fluid, showing the presence of R282W mutation in the peritoneal (ascitic) fluid cells but not in the bulky mass. l Sanger sequencing analysis confirming the presence of a heterozygous R282W mutation in the cells from peritoneal fluid, and lack of mutation in the bulky mass.

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