Skip to main content
Fig. 4 | Experimental Hematology & Oncology

Fig. 4

From: Analysis of the anti-proliferative and the pro-apoptotic efficacy of Syk inhibition in multiple myeloma

Fig. 4

a–c Inhibition of Syk induces apoptosis in MM cell lines. a–c Viability of MM cells was defined by the amount of nuclear fragmentation and compared to the respective DMSO control. MM cell lines [AMO-1 (a), U226 (b) and primary MM cells from patients with plasma cell leukemia (purity >90%) (c)] were treated with various concentrations of Syk inhibitors (Piceatannol, R406 and Bay61-3606) as indicated. After 24 h of incubation with Syk inhibitors, propidium iodide-staining was performed. Nuclear fragmentation was determined by flow cytometry. zVAD-FMK was added as confirmation for caspase activation (white bar graphs). c Primary MM cells were isolated from peripheral blood from patients with plasma cell leukemia (purity >90%) and analyzed as described above. Results are expressed as the mean of three panels per pattern. Results are from one experiment representative of at least three. The significance is related to DMSO as control. +P<1.000, *P < 0.1, **P < 0.01, ***P < 0.001.

Back to article page