Fig. 3

a–c Syk inhibition reduces phosphorylation of p38 and ERK1/2 in MM cell lines. a, b MM cell lines were treated with Piceatannol at concentrations of 10 and 25 µM. Phosphorylation status of down stream signaling mediators p38-Kinase and ERK1/2-Kinase (MAPK p42 and p44) was evaluated by Western blotting using antiphospho-p38 and antiphospho-ERK 1/2 antibodies. GAPDH was used to confirm equal amounts of protein. Results are representative of at least three independent experiments. c Nuclear translocation of NF-κB family members was determined by Western Blot analysis of nuclear extracts upon incubation with Syk inhibitors (c). Piceatannol was applied at 10 and 25 µM. DMSO was used as control. Proteins detected are indicated by an arrow on the right. Ponceau S staining was performed to confirm equal amounts of protein. Results are representative of at least three independent experiments.