Fig. 2
a, b Syk inhibitors reduce proliferation and migration in a dose-dependent manner. a MM cell lines were incubated with Syk inhibitors (Piceatannol or Bay61-3606) for 24 h and the proliferative response was analyzed by 3H-thymidine uptake. Syk inhibition resulted in a decrease of cell proliferation. b Migration of MM cells towards SDF-1α were performed with or without Syk inhibitors (Piceatannol, Bay61-3606 and R406) using transwell chemotaxis assays. The percentage of migrated cells was determined after 3 h by flow cytometry. Syk inhibitors diminished the migration towards SDF-1α stimulated cells. The shown results are representative for all tested MM cell lines. Results are expressed as the mean of three panels per pattern. Results are shown from one experiment representative of at least three. DMSO or Ampuwa was used as control. The significance is related to DMSO/Ampuwa. +P < 1.000, *P < 0.1, **P < 0.01, ***P < 0.001.