Proteomic analysis of HEK293 cells expressing non small cell lung carcinoma associated epidermal growth factor receptor variants reveals induction of heat shock response

Table 1 Differentially expressed proteins in cells expressing mutants vs. wild type EGFR identified by MALDI-TOF/MS analysis

Spot No.	Protein name	Acc. No	Mol. Wt	PI	Mascot Score	L858Rvs. WT	L861Q vs. WT	A871G vs. WT
6601	Heat shock cognate 71 kDa protein	P11142	71082	5.37	24	1.8	1.2	1.13
7501	Heat shock 70 kDa protein 1	P08107	70294	5.48	147	1.7	2.1	0.93
3702	Endoplasmin (GRP94) HSP90B1	P14625	92696	4.76	88	0.02	0.28	0.02
5503	60 kDa heat shock protein, mitochondrial	P10809	61187	5.7	75	1.4	1.2	1.03
4701	78 kDa GRP (HSP5A)	P11021	72402	5.07	158	1.1	1.31	0.34
7502	T-complex protein 1 subunit epsilon	P48643	60089	5.45	63	0.29	0.36	0.56
7403	Protein disulfide-isomerase	P30101	57146	5.98	84	0.73	0.96	0.63
4501	Tubulin alpha-1B chain	P68363	50804	4.94	128	0.58	0.69	0.33
3401	Tubulinbeta-2C chain	P68371	50255	4.79	239	0.63	1.2	0.72
4501	Vimentin	P08670	53676	5.06	24	0.58	0.69	0.33
6302	Keratin, type I cytoskeletal 18, 19	P05783	48029	5.34	319	0	1.5	0.86
7402	Keratin, type II cytoskeletal 8	P05787	53671	5.52	59	0.73	0.96	0.63
5203	Actin, cytoplasmic 2	P63261	42108	5.31	107	1.2	1.31	0.79

ISSN: 2162-3619