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Figure 3 | Experimental Hematology & Oncology

Figure 3

From: Lactate, a putative survival factor for myeloma cells, is incorporated by myeloma cells through monocarboxylate transporters 1

Figure 3

Induction of apoptosis in myeloma cells by modulation of MCT1. (A) Induction of apoptosis by CHC. The myeloma cell lines KMS-12PE (upper panels) and KMM-1 (lower panels) were treated with CHC at 0, 1.25, 2.5, or 5 mM for 24 h and then stained with Annexin V/PI for flow cytometry analysis. Treatment with CHC induces Annexin V-positive (open bars) and PI-positive (filled bars) populations in a dose-dependent manner. *p values for annexin V; **p values for PI. Representative raw data of the flow cytometry analyses are shown in the right panels. (B) CHC does not induce apoptosis in normal PBMCs. PBMCs were treated with CHC as described for (A), and do not undergo apoptosis. (C) CD138 positive cells obtained from a MM patient were treated with CHC at 0, 1.25, 2.5, or 5 mM for 24 h and then stained with Annexin V/PI for flow cytometry analysis. CHC induced apoptosis in myeloma cells obtained from a MM patient in a dose dependent manner. (D) MCT1 knockdown by siRNA results in induction of apoptosis. KMM-1 cells were treated with each siRNA and incubated for up to 5 days. On day 5, cells were subjected to Annexin V/PI staining. Amount of apoptotic cells are quantified and shown in the left panel. Results of FACS analysis are shown in the right panel. Knockdown of MCT1 shows significant induction of apoptosis.

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