Skip to main content
Figure 6 | Experimental Hematology & Oncology

Figure 6

From: Immunosuppressive mechanisms of human bone marrow derived mesenchymal stromal cells in BALB/c host graft versus host disease murine models

Figure 6

Chemokine expression in host spleen and target organs. (A) RANTES expression. (B) Expression of CCL3 and CXCL9. (C-D) Expression of CCR5, CXCR3 and CCR7. Lethally irradiated BALB/c host mice were given intravenous injections of 2 × 10^6 TCDBM cells from C57BL/6 donors with or without 0.25 × 10 ^6 CD4+ T cells from donors and 1 × 10 ^6 hMSCs. (A) Host spleen and colon were harvested 6d after transplantation and RANTES expression measured using ELISA (*p = 0.0356). Data were combined from 2 independent experiments with 3-4 mice in each experiment. (B) Expression of CCL3 and CXCL9 6d after transplantation in BALB/c host spleen, colon and lung were measured by RT-qPCR. Data were combined from 2 independent experiments with 4-6 mice. (C,D) Expression of CCR5, CXCR3 and CCR7 6d after transplantation in BALB/c host spleen were measured by using trypan blue cell exclusion assay and flow cytometry and expressed in absolute numbers of CD3+ CCR5+, CD3+ CXCR3+ (C) and CD3+ CCR7+ (D) splenocytes. Data were combined from 2 independent experiments with 2-4 mice in each experiment (*p<0.005).

Back to article page